Probe

Melting peak

Mean Tm

SD

N

Tm Acceptance Criteria


136A2

A^{136}

62.0°C

0.49

2228

60.0°C – 64.0°C

136A2

V^{136}

53.2°C

0.36

214

51.2°C – 55.2°C

136A2

T
^{136}

56.9°C

0.15

8

55.2°C – 58.9°C

136A2

AT
^{137}

59.5°C

0.19

7

59.0°C – 60.0°C

154H2

H^{154}

56.6°C

0.51

300

54.6°C – 58.7°C

154H2

R^{154}

49.9°C

0.29

2171

47.9°C – 51.9°C

171R6

R^{171}

55.4°C

0.39

1524

53.4°C – 57.4°C

171R6

Q^{171}

49.8°C

0.31

1392

48.4°C – 51.8°C

171R6

QE
^{175}

49.7°C

0.21

12

48.4°C – 51.8°C

171R6

L
^{168}
Q

47.9°C

0.19

5

46.9°C – 48.4°C

171R6

H^{171}

44.8°C

0.27

168

42.8°C – 46.8°C

171R6

K
^{171}

41.0°C

0.15

8

39.0°C – 42.8°C

141C3

F^{141}

53.9°C

0.19

39

51.9°C – 55.9°C

141C3

L^{141}

45.0°C

0.14

6

43.0°C – 47.0°C

 The mean Tms for each probe melting peak are presented, where N is the number of peaks investigated and SD is the standard deviation. The peak Tms for the major PRNP alleles were obtained from six 384 well plates prepared on different working days, thereby accounting for sampletosample and runtorun sources of variation. Variant alleles exhibiting uncommon genotypes or additional SNPs are italicised. Acceptance criteria for these rare polymorphisms were estimated from the few samples analysed, minimising the potential for miscalling melting peaks.