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Table 2 Probe Tms and acceptance criteria

From: Analysis of multiple single nucleotide polymorphisms closely positioned in the ovine PRNPgene using linear fluorescent probes and melting curve analysis

Probe Melting peak Mean Tm SD N Tm Acceptance Criteria
136A2 A136 62.0°C 0.49 2228 60.0°C – 64.0°C
136A2 V136 53.2°C 0.36 214 51.2°C – 55.2°C
136A2 T 136 56.9°C 0.15 8 55.2°C – 58.9°C
136A2 AT 137 59.5°C 0.19 7 59.0°C – 60.0°C
154H2 H154 56.6°C 0.51 300 54.6°C – 58.7°C
154H2 R154 49.9°C 0.29 2171 47.9°C – 51.9°C
171R6 R171 55.4°C 0.39 1524 53.4°C – 57.4°C
171R6 Q171 49.8°C 0.31 1392 48.4°C – 51.8°C
171R6 QE 175 49.7°C 0.21 12 48.4°C – 51.8°C
171R6 L 168 Q 47.9°C 0.19 5 46.9°C – 48.4°C
171R6 H171 44.8°C 0.27 168 42.8°C – 46.8°C
171R6 K 171 41.0°C 0.15 8 39.0°C – 42.8°C
141C3 F141 53.9°C 0.19 39 51.9°C – 55.9°C
141C3 L141 45.0°C 0.14 6 43.0°C – 47.0°C
  1. The mean Tms for each probe melting peak are presented, where N is the number of peaks investigated and SD is the standard deviation. The peak Tms for the major PRNP alleles were obtained from six 384 well plates prepared on different working days, thereby accounting for sample-to-sample and run-to-run sources of variation. Variant alleles exhibiting uncommon genotypes or additional SNPs are italicised. Acceptance criteria for these rare polymorphisms were estimated from the few samples analysed, minimising the potential for miscalling melting peaks.