Figure 1

SDS-PAGE Western blot of anthrax infected rabbit plasma showing PA ₂₀ and PA ₆₃ just prior to death. Lanes 1 to 4 were stained with anti-PA₂₀ specific MAb and lanes 5 to 8 stained with anti – PA63 specific MAb. Lanes 1 (arrow points to PA₁₇) and 5 were loaded with the LIST PA₁₇ at 5 mg/ml. Lanes 2 and 6 were left blank to minimize cross contamination. Lanes 3 (arrow points to PA₂₀) and 7 (arrow points to PA₆₃) were loaded with 1:20 dilution of plasma from Ames strain challenged rabbit #41 just prior to death. Lanes 4 (arrow points to PA₈₃) and 8 (arrow points to PA₈₃) were loaded with purified PA83 @ 10 mg/ml.