Figure 2From: Rapid Detection of Chlamydia trachomatisand Typing of the Lymphogranuloma venereum associated L-Serovars by TaqMan PCR Amplification charts and standard curves for the CT/LGV multiplexed real-time PCR. The linear range of the assay was determined using duplicates of 1.25 × [109, 107, 105, 103, 102, 101] copies of each cloned amplicon. The threshold values (Ct) were plotted against the corresponding copy numbers, and the efficiency, slope and linear regression correlation (r2) were calculated for each reaction by the Biorad IQ5 software.Back to article page