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Table 1 Comparison of phenotypic and molecular assays apllied to bloodculture bottles

From: Diagnosis of bacteremia in pediatric oncologic patients by in-house real-time PCR

  Phenotipyc detection and identification by phenotypic analisys (n = 124) Molecucar detection by GP/GN multiplex PCR Concordance (%) Molecular identification by specific-species qPCR (n = 124) Concordance (%) Average Cycle threshold
S. aureus 12 12 100 % 10 83.40 % 17,683
SCoN 35 28 80 % 32 91.40 % 20,608
Enterococcus spp. 5 5 100 % 5 100 % 24,923
S. pneumoniae 1 1 100 % 1 100 % 20,977
K. pneumoniae 3 3 100 % 3 100 % 29,111
E. cloacae 6 6 100 % 6 100 % 20,919
E. coli 8 7 87.50 % 7 87.50 % 18,995
S. marcescens 2 2 100 % 2 100 % 25,252
P. aeruginosa 14 13 92.80 % 13 92.80 % 19,395
S. malthofilia 1 1 100 % 1 100 % 36,974
Acinetobacter spp. 1 1 100 % 1 100 % 27,993
Candida spp. 12 12 100 % 3 25 % 25,464
Other fungi 2 2 100 % a a a
Streptococcus sp. 9 7 77.80 % a a a
Others 13 12 92.30 % a a a
  1. aSpecies not included in the in-house qPCR standardized assay described in this study