Table 1 Comparison of phenotypic and molecular assays apllied to bloodculture bottles
From: Diagnosis of bacteremia in pediatric oncologic patients by in-house real-time PCR
|  | Phenotipyc detection and identification by phenotypic analisys (n = 124) | Molecucar detection by GP/GN multiplex PCR | Concordance (%) | Molecular identification by specific-species qPCR (n = 124) | Concordance (%) | Average Cycle threshold |
|---|---|---|---|---|---|---|
S. aureus | 12 | 12 | 100Â % | 10 | 83.40Â % | 17,683 |
SCoN | 35 | 28 | 80Â % | 32 | 91.40Â % | 20,608 |
Enterococcus spp. | 5 | 5 | 100Â % | 5 | 100Â % | 24,923 |
S. pneumoniae | 1 | 1 | 100Â % | 1 | 100Â % | 20,977 |
K. pneumoniae | 3 | 3 | 100Â % | 3 | 100Â % | 29,111 |
E. cloacae | 6 | 6 | 100Â % | 6 | 100Â % | 20,919 |
E. coli | 8 | 7 | 87.50Â % | 7 | 87.50Â % | 18,995 |
S. marcescens | 2 | 2 | 100Â % | 2 | 100Â % | 25,252 |
P. aeruginosa | 14 | 13 | 92.80Â % | 13 | 92.80Â % | 19,395 |
S. malthofilia | 1 | 1 | 100Â % | 1 | 100Â % | 36,974 |
Acinetobacter spp. | 1 | 1 | 100Â % | 1 | 100Â % | 27,993 |
Candida spp. | 12 | 12 | 100Â % | 3 | 25Â % | 25,464 |
Other fungi | 2 | 2 | 100Â % | a | a | a |
Streptococcus sp. | 9 | 7 | 77.80Â % | a | a | a |
Others | 13 | 12 | 92.30Â % | a | a | a |