Fig. 4
Ex vivo MGIA using M. smegmatis distinguishes between immunized and control animals. 5 × 106 splenocytes from mice immunized with BCG Pasteur Aeras (grey circles) or given saline (open squares) were co-cultured with 50 CFU of M. smegmatis 5 weeks after immunization. Splenocytes were obtained from 6 immunized and 6 control animals, each represented by an individual data point. Error bars represent the median +/- interquartile range. Statistical significance was tested using the unpaired t test function in GraphPad Prism