Skip to main content

Table 1 Oligonucleotide primers and probe for LF-RPA assay

From: Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid

Name

Sequence (5’→3’)

CM ITS P4

FITC-TACACAAACTTCTAAATGTAATGAATGTAATC(H)TATTATAACA ATAATAAA-P

CM ITS F4

TGAACTGTTTATGTGCTTCGGCACGTTTTAC

CM ITS R4

Biotin-TCGATGTGGAAGCCAAGAGATCCGTTGTTG

  1. CM: cryptococcal meningitis; ITS: internal transcribed spacer of ribosomal RNA gene; F: forward primer; R: reverse primer; P: probe; FITC: fluorescein isothiocyanate; H: tetrahydrofuran spacer; P: 3’ phosphate to block elongation.
Back to article page

BMC Infectious Diseases

ISSN: 1471-2334

Contact us