Table 2 Immunization and sampling schedule of BALB/c mice
Weeks | 0 W | 2 W | 4 W | 6 W | 8 W | 10 W | 20 W | |
|---|---|---|---|---|---|---|---|---|
Groups | Action | Pre-immunization analysis | Analysis | Boost1 & analysis | Analysis | Boost 2 & analysis | Analysis | Analysis |
 1 (n = 12) 800 ng HCVp6 | 800 ng ELISA & EliSpot | ELISA & EliSpot | 800 ng ELISA, EliSpot & Neutralization | ELISA & EliSpot | 800 ng ELISA & EliSpot | ELISA & EliSpot | EliSpot | |
 2 (n = 12) 1600 ng HCVp6 | 1600 ng ELISA & EliSpot | ELISA & EliSpot | 1600 ng ELISA, EliSpot & Neutralization | ELISA & EliSpot | 1600 ng ELISA & EliSpot | ELISA & EliSpot | EliSpot | |
 3 (n = 12) 16 μg HCVp6 | 16 μg ELISA & EliSpot | ELISA & EliSpot | 16 μg ELISA, EliSpot & Neutralization | ELISA & EliSpot | 16 μg ELISA & EliSpot | ELISA & EliSpot | EliSpot | |
 control (n = 12) PBS 120 ul | PBS ELISA & EliSpot | ELISA & EliSpot | PBS ELISA, EliSpot & Neutralization | ELISA & EliSpot | PBS ELISA & EliSpot | ELISA & EliSpot | EliSpot | |