Fig. 5

Phylogenetic relationships of P. vivax and P. falciparum isolates from Northern and Southern Punjab. Plasmodium vivax clustered into four and P. falciparum clustered into two sub-clusters within the main clade. The neighbor-joining method was applied to infer evolutionary history. The branch length = 6.06796875 was shown and the percentage of replicating trees was shown next to the branches. The p-distance method was used to evaluate evolutionary distance and it was in the units of the number of base differences per site. The analysis involved 21 nucleotides DNA sequences