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Table 3 Flow cytometry-based detection (MFI) of flavivirus envelope protein in subpopulations of human peripheral blood mononuclear cells after ex-vivo infectiona

From: Zika virus infects human blood mononuclear cells

Donors

PBMC subpopulations (MFI)

CD3+CD4+4G2+

CD3+CD8+4G2+

CD3CD19+4G2+

CD14+4G2+

D1

201.0

194.00

189.0

266.0

D3

142.0

135.00

167.0

zero

D4

2.0

zero

374.0

NDb

D5

27.0

12.00

42.0

NDb

D7

19.0

zero

zero

zero

D9

zero

zero

zero

123.0

D14

zero

zero

zero

6.0

D15

zero

zero

27.0

zero

D16

zero

44.00

zero

zero

  1. aMFI (Median of fluorescence intensity) was calculated by subtracting the median of 4G2 labeling in PBMCs subpopulations who received ZIKV as compared with the background 4G2 labeling profile in PBMCs subpopulations from the same donor, but that were not exposed to ZIKV (MOCK). Donors D2, D6, D8, D10, D11, D12 and D13 cells were all negative for 4G2 staining. bND = not determined, as very low amounts of these subpopulations were detected