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Table 1 CSF microscopy, culture and PCRctic results in universal containers (samples 1–52) and snap-top tubes (samples 53–73)

From: Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

  

Microscopy

 

Bacteriology

No

WCCa

RCCb

R/Wc

Culture

PCRctic

Universal container

1

0

0

 

no growth

negative

2

10

662

 

no growth

negative

3

26

30,780

1183

no growth

Armatimonadetes

4

12

6930

 

no growth

negative

5

6

32,310

 

no growth

negative

6

?

?

 

no growth

negative

7

0

9180

 

no growth

negative

8

0

260

 

no growth

Flavobacteriaceae [23] & Rhodobacteraceae

9

0

6570

 

no growth

Hymenobacter (Flavobacteriaceae)

10

0

0

 

no growth

negative

11

24

51,120

2130

no growth

Flavobacteriaceae

12

6

280

 

no growth

Ochrobactrum sp. [24]

13

8

54,000

 

no growth

Mixed sequence

14

0

137

 

no growth

Oscillatoriales

15

0

690

 

no growth

negative

16

8

12

 

no growth

Acinetobacter [25]

17

24

20

0.8

no growth

negative

18

22

42

1.9

no growth

negative

19

2

4680

 

no growth

negative

20

14

15,840

 

no growth

negative

21

0

24

 

no growth

negative

22

6

52

 

no growth

Sphingomonadaceae [26]

23

8

14

 

no growth

Aerococcus christensenii [27] & Sneathia amnii [28]

24

12

228

 

no growth

negative

25

0

2610

 

no growth

negative

26

0

140

 

no growth

negative

27

10

300

 

no growth

negative

28

30

11,430

381

no growth

Sneathia amnii

29

0

3310

 

no growth

negative

30

0

52

 

no growth

Streptococcaceae [29,30,31]

31

0

2

 

no growth

negative

32

4

146

 

no growth

negative

33

16

22,860

 

no growth

Pseudomonas sp. [12,13,14]

34

0

9720

 

no growth

Bdellovibrio

35

0

10,440

 

no growth

Staphylococcus [12,13,14]

36

8

49,500

 

no growth

negative

37

8

1000

 

no growth

Ureaplasma [32]

38

0

790

 

no growth

negative

39

0

1800

 

no growth

negative

40

0

40

 

no growth

negative

41

0

80

 

no growth

negative

42

0

24,800

 

no growth

negative

43

4

1126

 

no growth

negative

44

0

38

 

no growth

negative

45

10

7560

 

no growth

Cloacibacterium rupense

46

8

9360

 

no growth

negative

47

4

284

 

no growth

Mixed sequence

48

0

18,900

 

no growth

negative

49

0

0

 

no growth

negative

50

0

6

 

no growth

Methylobacterium sp.

51

4

10,170

 

no growth

Pedobacter suwonensis

52

0

0

 

no growth

Staphylococcus aureus

Snap-top tube (“Eppendorf”)

53

16

22,860

 

no growth

negative

54

0

40

 

no growth

negative

55

2

70

 

no growth

negative

56

0

3420

 

no growth

negative

57

0

0

 

no growth

negative

58

2

2088

 

no growth

negative

59

42

21,690

516

no growth

negative

60

0

1080

 

no growth

negative

61

0

40

 

no growth

negative

62

0

132

 

no growth

negative

63

12

22

 

no growth

Geobacter

64

4

16

 

no growth

negative

65

10

1940

 

no growth

negative

66

8

4

 

no growth

negative

67

4

36

 

no growth

Mixed sequence

68

0

2

 

no growth

negative

69

0

8820

 

no growth

negative

70

0

8010

 

no growth

negative

71

6

4

 

no growth

negative

72

51

406,800

7976

no growth

negative

73

6

12

 

no growth

negative

  1. a WCC – CSF White blood cell count, per microlitre
  2. b RCC – CSF red blood cell count, per microlitre
  3. c R/W – the ratio of CSF red to white cells (calculated only for samples with ≥20 white cells in microliter [10]. The ratio that allows accurate exclusion of neonatal bacterial meningitis remains uncertain [8]