Table 1 Primers and probes used
Target gene | Oligonucleotide sequence (5′-3′) | Reference |
|---|---|---|
glyA | CATCTTCCCTAGTCAAGCCT | Vandamme, et al. [29] |
AAGATATGGCTCTAGCAAGAC | ||
gyrA | TGCTGTTATAGGTCGTTA | This study |
CCTTGTCCTGTAATACTTG | ||
gyrA Wild type | HEX 5′-CCCACATGGAGATACAGCAGTTTATG-3′-BHQ2 | This study |
gyrA point mutation T86I | 6-FAM 5’CCCACATGGAGATATAGCAGTTTATG-3’BHQ1 | This study |
gyrA | GCCTGACGCAAGAGATGGTT | Bakeli, et al. [17] |
TTTGTCGCCATACCTACAGC |