Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni

Table 2 Susceptibility of 189 C. jejuni isolates, and point mutation T86I of the gyrA gene

Phenotype	No. of Isolates	CIP_Disk diffusion	NA_Disk diffusion	CIP_MIC	Real-Time PCR
Phenotype	No. of Isolates	Range (mm)	Range (mm)	Range (μg/mL)	*gyrA* gene (n)
CIP-S and NA-S	44	29–50	19–37	0.008–1.0	Wild type 44
CIP-R and NA-R	141	6–11	6–9	4–32	T86I mutation 141
CIP-S and NA-R	4	24–38	6	0.047–0.19	Wild type 4

CIP-S ciprofloxacin sensitive, CIP-R ciprofloxacin resistant, NA-S nalidixic acid sensitive, NA-R nalidixic acid resistant, MIC minimal inhibitory concentration

ISSN: 1471-2334