Evaluation of digital PCR assay in detection of M.tuberculosis IS6110 and IS1081 in tuberculosis patients plasma

Table 3 Diagnostic performance of plasma IS6110- and IS1081- dPCR assay for Disseminated TB

	Cut-off point (copies/20 μl)	AUC	Sensitivity(%)	Specificity(%)	LR+	LR-	PPV(%)	NPV(%)
Disseminated TB (16) compared with HC (n = 106)
IS6110-dPCR	16	0.98 (0.97–1.00)	77.8 (47.6–92.7)	99.1 (94.9–100.0)	86.1 (12.1–614.4)	0.2 (0.1–0.5)	92.9 (64.7–99.9)	97.2 (92.1–99.4)
IS1081-dPCR	14	0.95 (0.91–0.99)	37.5 (16.2–64.6)	99.1 (94.9–100.0)	39.8 (5.1–309.0)	0.6 (0.4–0.9)	85.7 (38.1–99.8)	91.3 (84.6–95.8)

AUC area under receiver operating characteristic (ROC) curve, LR+ positive likelihood ratio, LR- negative likelihood ratio, PPV positive predictive value, NPV negative predictive value

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