Table 6 Performance characteristics of the three tests in Melong HD. Summary of sensitivity, specificity, PPV and NPV for Microscopy, actin-qPCR and O-150 LAMP at different time points following direct observed treatment of microfilaridermic individuals with ivermectin in Melong Health District

45.4 (10.1–91.2)

99.1 (89.7–100)

89.6 (29.3–100)

91.1 (71.0–99.2)

87.6 (32.2–100)

98.7 (87.1–100)

92.2 (36.2–100)

98.0 (78.3–100)

94.2 (52.0–100)

89.3 (71.5–99.7)

60.5 (18.4–99.3)

98.9 (87.4–100)

90.6 (48.3–100)

94.7 (82.3–100)

78.9 (35.2–99.9)

98.0 (82.0–100)

38.0 (10.9–80.6)

99.4 (92.9–100)

93.1 (43.4–100)

87.8 (69.8–97.9)

92.1 (55.3–100)

89.8 (75.9–99.7)

66.1 (28.1–99.2)

98.1 (84.9–100)

88.9 (58.1–99.9)

96.1 (84.9–99.8)

87.5 (59.2–99.4)

96.5 (84.5–100)

96.7 (69.9–100)

98.8 (89.2–100)

96.2 (66.7–100)

99.0 (89.4–100)

97.1 (74.0–100)

75.3 (58.7–88.5)

55.0 (30.6–78.2)

98.9 (87.7–100)

80.2 (57.2–95.0)

95.9 (89.4–99.2)

81.5 (56.6–96.4)

95.7 (87.9–99.0)

78.3 (53.3–97.9)

99.7 (96.5–100)

98.2 (82.1–100)

95.4 (87.4–99.7)

98.5 (84.3–100)

84.7 (75.6–92.3)

58.7 (39.1–79.4)

99.6 (95.4–100)