Describing nearly two decades of Chagas disease in Germany and the lessons learned: a retrospective study on screening, detection, diagnosis, and treatment of Trypanosoma cruzi infection from 2000 – 2018

Table 1 German centres with CD diagnostics for routine patient care during 2000–2018

Center	City	Type of test	Test availability (years)	Number of retrieved tests	Data availability (years)
Institute of Tropical Medicine and International Health, Charité – Universitätsmedizin in Berlin	Berlin	in-house IFT	2000–2010	204	2002–2018
	Berlin	in-house ELISA	2000–2018	830	2002–2018
Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn	Bonn	in-house IFT	2000–2010	201	2004–2018
		commercial ELISA	2011–2018	190
		commercial RDT	2000–2010	116
Bernhard Nocht Institute for Tropical Medicine	Hamburg	in-house IFT	2000–2018	2641	2000–2018^a
		in-house ELISA	2000–2018	2654
		PCR^b	2000–2018	200
Division of Infectious Diseases and Tropical Medicine, University Hospital, LMU Munich	Munich	in-house IFT	2000–2018	1670	2000–2018
		in-house ELISA	2000–2018	1668
		PCR^b	2013–2018	35
Institute of Tropical Medicine, Medical Department, University Hospital Tübingen	Tuebingen	in-house IFT	2009–2018	319	2009–2018
			Total	10,728

CD Chagas disease, ELISA Enzyme-linked immuno-sorbent assay, IFT Immunofluorescence test, PCR Polymerase chain reaction, RDT Rapid diagnostic test
^aIndividual positive tests 2000–2018 and negative tests 2010–2018 could be retrieved
^bHamburg offered a conventional PCR that was followed up by sequencing of the PCR product if positive, whereas Munich performed a conventional PCR that was followed up with an independent commercially available qPCR if positive (for details please see Supplement S2)

ISSN: 1471-2334