Table 1 PCR primers sequences
From: Increased mRNA expression of key cytokines among suspected cases of Pneumocystis jirovecii infection
Gene name | Primer sequence | Annealing temperature | Product length (bp) |
|---|---|---|---|
IL - 2 | FP: 5′-AACCTCAACTCCTGCCACAA-3′ | 60 °C | 197 |
RP: 5′-GCATCCTGGTGAGTTTGGGA-3′ | |||
IL - 4 | FP: 5′-CACAACTGAGAAGGAAACCTTCTG-3′ | 60 °C | 253 |
RP: 5′-CTCTCTCATGATCGTCTTTAGCCTTTC-3′ | |||
IL - 10 | FP: 5′-AGAACCT GAAGACCCTCAGGC-3’ | 60 °C | 78 |
RP: 5′-CCACGGCCTTGCTCTTGTT-3’ | |||
IL - 13 | FP: 5′-GCTCCTCAATCCTCTCCTGTT-3’ | 58 °C | 485 |
RP: 5′-GCAACTTCAATAGTCAGGTCC-3’ | |||
GAPDH | FP: 5′-GAAGGTGAAGGTCGGAGTC-3’ | 60 °C | 226 |
RP: 5′-GAAGATGGTGATGGGATTTC-3’ |