Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

Table 1 Primers for uropathogenic Escherichia coli virulence genes PCR assay [12, 16]

Virulence factor	Target gene(s)	Primer Name	Primer Sequence (5′- 3′)	Size of amplicon (bp)
Type 1 fimbriae	fimH	fimH-f	5′-AACAGCGATGATTTCCAGTTTGTGTG-3′	465
Type 1 fimbriae	fimH	fimH-r	5′-ATTGCGTACCAGCATTAGCAATGTCC-3′	465
P fimbriae	papC	pap1	5′-GACGGCTGTACTGCAGGGTGTGGCG-3’	328
P fimbriae	papC	pap2	5′-ATATCCTTTCTGCAGGGATGCAATA-3’	328
S and FIC fimbriae	Sfa/focDE^h region	sfa1	5′-CTCCGGAGAACTGGGTGCATCTTAC-3’	410
S and FIC fimbriae	Sfa/focDE^h region	sfa2	5′-CGGAGGAGTAATTACAAACCTGGCA-3’	410
Afa adhesins	afaC^c	afa-f	5′-CGGCTTTTCTGCTGAACTGGCAGGC-3’	672
Afa adhesins	afaC^c	afa-r	5′-CCGTCAGCCCCCACGGCAGACC-3’	672
Hemolysin	hlyCA region	hly s	5′-AGATTCTTGGGCATGTATCCT-3’	556
Hemolysin	hlyCA region	hly as	5′-TTGCTTTGCAGACTGTAGTGT-3’	556
Cytotoxic necrotizing factor	cnf	cnf s	5′-TTATATAGTCGTCAAGATGGA-3’	693
Cytotoxic necrotizing factor	cnf	cnf as	5′-CACTAAGCTTTACAATATTGA-3’	693
Aerobactin	iucC	aer s	5′-AAACCTGGCTTACGCAACTGT-3’	269
Aerobactin	iucC	aer as	5′-ACCCGTCTGCAAATCATGGAT-3’	269

ISSN: 1471-2334