Fig. 2

A agarose gel electrophoresis (1%) of PCR amplified AMOS- PCR products from isolated bacteria samples. Lane M indicated DNA marker (1000 bp DNA ladder). Lane 1 shows reference bacteria of B. abortus 544; Lane 2 shows reference bacteria of B. melitensis 16 M; Lane 3 corresponds to wild type of B. melitensis ; Lane 4 and 5 shows B. abortus from extracted DNA of isolated bacteria from disc culture. B agarose gel electrophoresis (1%) of PCR amplified Bruce-ladder PCR products from DNA bacterial samples. Lane 1 shows DNA marker (1000 bp DNA ladder). Lane 2 corresponds to reference bacteria of B. abortus RB51; Lane 3 shows reference bacteria of B. melitensis Rev1; Lane 4 shows reference bacteria of B. melitensis 16 M; Lane 5 illustrates reference bacteria of B. abortus 544; Lane 6 negative control, Lane 7 and 8 corresponds to isolated of isolated bacteria from disc culture. C colony of B. abortus on selective medium