Table 2 Microbiological testing on different days in relation to the day of diagnosis
Sample type | Day of testing | Pathogen | Analysis | Result | |
|---|---|---|---|---|---|
Molecular testing and culture | Vesicle secretion | d1 | Monkeypox virus | PCR | Positive |
d12 | Herpes simplex virus (HSV) type 1 and 2 | Negative | |||
Throat swab and urine | C. trachomatis and N. gonorrhoeae | Negative | |||
CSF | d12 | Bacteria | Culture | Negative | |
HSV type 1 and 2, varicella zoster virus | PCR | Negative | |||
d12, d15 | Monkeypox virus | Negative | |||
Orthopox virus | Negative | ||||
E. coli K1, H. influenzae, L. monocytogenes, N. meningitidis (capsuled), S. agalactiae, S. pneumoniae, cytomegalovirus, enterovirus, HSV type 1 and 2, human herpes virus 6, human parechovirus, varicella-zoster-virus and Cryptococcus neoformans/gattii. | FilmArray | Negative | |||
Serological testing | CSF | d12 | Monkeypox virus | Immunfluorescence (titer IgG) | 32 1280 |
Serum | d13 | ||||
CSF | d12 | EBV | Immunfluorescence (titer IgG) | 0 | |
Serum | d13 | EBV | 80 | ||
Serum | d13 | HIV | Screening (antigen and antibodies) | Negative | |
Antigen innotest | Negative | ||||
Hepatitis B | HBsAg | Negative | |||
Hepatitis C | Anti HCV | Negative | |||
CMV, EBV | CMV IgG | Positive | |||
CMV IgM | Negative | ||||
EBV EBNA IgG | Positive | ||||
CSF | d12 | Borrelia Burgdorferi | IgG | Negative | |
IgM | Negative | ||||
Serum | d14 | IgG | Negative | ||
IgM (Screening) | Borderline positive | ||||
IgM (Western Blot) | Negative | ||||
CSF | d12 | Autoimmune encephalitis panel | antibodies against NMDA-R, CASPR2, AMPA1/2, LGI1, DPPX, GABARB1/2 | Negative | |
Serum | d13 | ||||
CSF | d12 | Tick-borne encephalitis | Intrathecal antibodies | Negative | |
Serum | d15 | TBE IgG | Positive | ||
TBE IgM | Negative | ||||
CSF | d15 | Syphilis | TPPA | Positive (serum/CSF quota 1280)* | |
VDRL | Negative | ||||
Serum | TPPA | Antibodies positive* | |||
VDRL | Negative | ||||
Wasserman’s reaction | Negative |